Journal: Cancer immunology research
Article Title: KIR3DL3 is an inhibitory receptor for HHLA2 that mediates an alternative immunoinhibitory pathway to PD1
doi: 10.1158/2326-6066.CIR-20-0315
Figure Lengend Snippet: (A) Jurkat IL2-reporter T cells expressing KIR3DL3 were co-cultured with CHO cells expressing anti-CD3 scFV, CHO cells coexpressing anti-CD3 scFV and HHLA2, or untransfected CHO cells in the presence or absence of CD28 mAb as indicated. Luciferase activity represented as relative light units (RLU). (B, C) Jurkat IL2-reporter T cells expressing KIR3DL3 were co-cultured with CHO cells coexpressing anti-CD3 scFV and HHLA2 in the presence of CD28 mAb and (B) HHLA2 mAbs or (C) KIR3DL3 mAbs. Fold activation of IL2 reporter luciferase activity is presented as mean ± S.D. (n≥3; **** P≤0.0001).
Article Snippet: Jurkat cells (clone E6–1) expressing IL2_promoter Luc_pcDNA (BPS Bioscience; cat. #60481) were transfected with KIR3DL3 (pEF-Puro) by electroporation.
Techniques: Expressing, Cell Culture, Luciferase, Activity Assay, Activation Assay